The measurement of endogenous carbon monoxide production.
نویسنده
چکیده
Recent findings that heme oxygenase-1 can be induced by oxidative stress and inflammation in many different cellular systems, and that carbon monoxide (CO) produced as a by-product of this enzyme is a signaling molecule, have generated a major research area with hundreds of studies published over the last few years. The measurement of expired CO concentration has been used in humans as a biomarker of induced heme oxygenase resulting from inflammation or oxidative stress, but a precise method of measuring endogenous CO production that can be easily used to study patients is needed. The present study describes such a method. The described method allows calculation of the rate of heme catabolism with a precision of ±2 μmol/h, ∼10% of the mean normal rate in subjects used in this investigation. This method, which is subject-patient friendly, precise, and inexpensive to perform, should be applicable to studies performed on humans with induced heme oxygenase and studies of effects of therapy for inflammatory and hemolytic diseases.
منابع مشابه
Endogenous carbon monoxide production in extracorporeal membrane oxygenation‐related hemolysis: potential use of point‐of‐care CO‐oximetry carboxyhemoglobin to detect hemolysis
Extracorporeal membrane oxygenation (ECMO)-related hemolysis is a rare but devastating condition. Death is inevitable without early recognition and prompt management. Endogenous carbon monoxide production, as an epiphenomenon of hemolysis, potentially allows rapid detection of such condition by use of point-of-care CO-oximetry carboxyhemoglobin.
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عنوان ژورنال:
- Journal of applied physiology
دوره 112 11 شماره
صفحات -
تاریخ انتشار 2012